Heterologous Gene Expression in Chlamydomonas reinhardtii Chloroplast by Heterologous Promoters and Terminators, Intercistronic Expression Elements and Minichromosome
Yunling Guo, Hui Xiong, Qiuling Fan, Deqiang Duanmu · 2024
Read Heterologous Gene Expression in Chlamydomonas reinhardtii Chloroplast by Heterologous Promoters and Terminators, Intercistronic Expression Elements and Minichromosome by Yunling Guo, Hui Xiong, Qiuling Fan, Deqiang Duanmu (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11650887.txt.
Description
Yunling Guo and three co-authors test genetic parts for algae bioengineering in a 2024 paper. A model green alga is a favorite host for making foreign proteins in its chloroplast. Expressing several genes at once, at high levels, still frustrates researchers.
The team shot DNA into chloroplasts with a particle gun and compared control parts from tobacco and the alga itself. Tobacco start signals failed to drive production, while tobacco stop signals worked fine. One algal connector element strongly boosted the gene placed after it. A tobacco connector proved useless in this host. A bacterial mini-chromosome system adds another route for carrying genes. The comparison gives builders a parts list that actually works in algal chloroplasts.
Catalog record
| CREATOR | Yunling Guo, Hui Xiong, Qiuling Fan, Deqiang Duanmu |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 32 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |