DNA Hybridization Kinetics Observed at the Single-Molecule Level Using Graphene Near-Field Effects
Maria · 2024
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Description
Soares, Freitas, Queirós, Purwidyantri, Alpuim, and Nieder watch single DNA molecules pair up in real time. Their sensor is a sheet of graphene carrying fluorophore-labeled DNA hairpins. Graphene quenches the labels in a distance-dependent way, so hybridization changes the light signal as hairpins move. A wide-field fluorescence microscope with a sensitive camera captured signals from single hairpins at low target concentration.
The team used the signals for super-resolution localization of each hairpin and measured how fast single hairpins unfold, at about 7 seconds. Some molecules paused in partly hybridized states, which more than doubled the unfolding time. A semiempirical model helped explain the observed kinetics.
Catalog record
| CREATOR | Maria |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 36 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |