An Open‐Source Tool for Investigation of Differential RNA Expression Between Spinal Cord Cells of Male and Female Mice
Justin Bellavance, Laurence S David, Michael E Hildebrand · 2024
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Description
Justin Bellavance, Laurence David, and Michael Hildebrand build an open source tool to compare gene activity in spinal cord cells of male and female mice. Chronic pain differs by sex in type, prevalence, and severity, yet spinal cord gene data had not been compared by sex at single cell scale. The team merges three large single nucleus datasets.
Machine learning classifies more than 37,000 cells into predicted types. Standard tests then find genes that differ by sex in all cells, in neurons, and in surface dorsal horn neurons that process pain. Differences are large, and stronger in inhibitory than in excitatory pain neurons. All results sit in a free web app, with enrichment analysis to guide new pain research.
Catalog record
| CREATOR | Justin Bellavance, Laurence S David, Michael E Hildebrand |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 42 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |