Metabolic Engineering of Komagataella phaffii for Xylose Utilization from Cellulosic Biomass
Jongbeom Park, Sujeong Park, Grace Evelina, Sunghee Kim, Yong-Su Jin, Won-Jae Chi, In Jung Kim, Soo Rin Kim, Xuebing Zhao · 2024
Read Metabolic Engineering of Komagataella phaffii for Xylose Utilization from Cellulosic Biomass by Jongbeom Park, Sujeong Park, Grace Evelina, Sunghee Kim, Yong-Su Jin, Won-Jae Chi, In Jung Kim, Soo Rin Kim, Xuebing Zhao (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11643697.txt.
Description
Jongbeom Park and colleagues engineer a yeast to eat xylose from plant biomass. Their study from twenty twenty-four rewrites Komagataella phaffii metabolism. Plant hydrolysates hold plenty of glucose and xylose sugars. This industrial yeast could not use xylose effectively before.
Three pathway genes were added with tuned promoter strengths. A strong promoter drove the middle gene of the pathway. Weaker promoters balanced the first and third genes. The engineered strain grew far better on kenaf hydrolysate. Growth beat the native strain by a clear margin. This is the first xylose pathway reported in this yeast. Biofactories could make protein and chemicals from crop waste.
Catalog record
| CREATOR | Jongbeom Park, Sujeong Park, Grace Evelina, Sunghee Kim, Yong-Su Jin, Won-Jae Chi, In Jung Kim, Soo Rin Kim, Xuebing Zhao |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 29 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |