Development of a Multiplex RT–qPCR Method for the Identification and Lineage Typing of Porcine Reproductive and Respiratory Syndrome Virus
Chunhao Tao, Xizhou Zhu, Ying Huang, Weifeng Yuan, Zhen Wang, Hongfei Zhu, Hong Jia, Dominika Marta Stygar, Barbara Bażanów · 2024
Read Development of a Multiplex RT–qPCR Method for the Identification and Lineage Typing of Porcine Reproductive and Respiratory Syndrome Virus by Chunhao Tao, Xizhou Zhu, Ying Huang, Weifeng Yuan, Zhen Wang, Hongfei Zhu, Hong Jia, Dominika Marta Stygar, Barbara Bażanów (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11642648.txt.
Description
Chunhao Tao and co-authors build, in 2024 work, a one-step lab test that both finds a pig virus and names its strain. The virus cuts sow births and fills piglets' lungs, and it comes in two great types, one of which splits into five lines that all roam China. Knowing the line guides farm control.
Seven probe sets were built: two aimed at a shell gene to split the types, and five at a second gene to name the lines. Packed into two pools, the test ran true, with tight repeat scores and reach down to a few copies per drop. On more than a thousand farm samples it matched the check method well. All found cases proved to be one line, proofed by letter reads.
Catalog record
| CREATOR | Chunhao Tao, Xizhou Zhu, Ying Huang, Weifeng Yuan, Zhen Wang, Hongfei Zhu, Hong Jia, Dominika Marta Stygar, Barbara Bażanów |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 39 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |