Moving Beyond Oxford Nanopore Standard Procedures: New Insights from Water and Multiple Fish Microbiomes
Ricardo Domingo-Bretón, Federico Moroni, Socorro Toxqui-Rodríguez, Álvaro Belenguer, M Carla Piazzon, Jaume Pérez-Sánchez, Fernando Naya-Català, Emina Pustijanac · 2024
Read Moving Beyond Oxford Nanopore Standard Procedures: New Insights from Water and Multiple Fish Microbiomes by Ricardo Domingo-Bretón, Federico Moroni, Socorro Toxqui-Rodríguez, Álvaro Belenguer, M Carla Piazzon, Jaume Pérez-Sánchez, Fernando Naya-Català, Emina Pustijanac (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11641276.txt.
Description
Ricardo Domingo-Breton and co-authors benchmark nanopore sequencing choices for fish farm microbes in a 2024 study, based on the head byline. The portable sequencing method profiles farm water and fish microbes fast, yet its many method options were not fully tested side by side. Library kits, primer spans, and basecalling accuracy modes were compared on water, feces, skin, and gut mucus samples.
Some sample types amplified only with the slower library kit and longer primers. Full length primers gave the deepest naming of microbes, though database matching ran thinner for some groups. The most accurate basecaller gave the cleanest reads but took far longer. The study tunes the full workflow for farm use.
Catalog record
| CREATOR | Ricardo Domingo-Bretón, Federico Moroni, Socorro Toxqui-Rodríguez, Álvaro Belenguer, M Carla Piazzon, Jaume Pérez-Sánchez, Fernando Naya-Català, Emina Pustijanac |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 50 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |