Development of a Rapid Visual Detection Assay for Duck Tembusu Virus Using RT-LAMP-CRISPR/Cas12a

Jimin Chen, Dagang Tao, Fan Yang, Chengfu Pan, Xinguo Bao, Shengsong Xie, Ping Gong, Changzhi Zhao, Ruiyi Lin, Fabio Ostanello · 2024

Read Development of a Rapid Visual Detection Assay for Duck Tembusu Virus Using RT-LAMP-CRISPR/Cas12a by Jimin Chen, Dagang Tao, Fan Yang, Chengfu Pan, Xinguo Bao, Shengsong Xie, Ping Gong, Changzhi Zhao, Ruiyi Lin, Fabio Ostanello (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11640670.txt.

Description

A team including Jimin Chen builds a fast field test for a duck virus that costs China's poultry farms dearly. The test pairs a copying method that works at one steady heat with a gene tool that flags the virus's code. Results show by eye, with no lab machine needed. The test found the virus in samples in under one hundred minutes and could spot very small amounts.

On duck embryos and chicken cells, its calls matched the standard lab test. The paper presents the assay as a handy on-site tool for early control of the disease.

Catalog record

CREATORJimin Chen, Dagang Tao, Fan Yang, Chengfu Pan, Xinguo Bao, Shengsong Xie, Ping Gong, Changzhi Zhao, Ruiyi Lin, Fabio Ostanello
YEAR2024
COLLECTIONPAPERS
FORMATTXT
FILE SIZE43 KB
VERSION1.0
LICENSECC BY 4.0
RIGHTSCC BY
SOURCEEurope PMC

Source: https://europepmc.org/articles/PMC11640670

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