Proteomic Analysis Reveals Cadherin, Actin, and Focal Adhesion Molecule-Mediated Formation of Cervical Cancer Spheroids
Piyatida Molika, Kittinun Leetanaporn, Wararat Chiangjong, Pongsakorn Choochuen, Raphatphorn Navakanitworakul · 2024
Read Proteomic Analysis Reveals Cadherin, Actin, and Focal Adhesion Molecule-Mediated Formation of Cervical Cancer Spheroids by Piyatida Molika, Kittinun Leetanaporn, Wararat Chiangjong, Pongsakorn Choochuen, Raphatphorn Navakanitworakul (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11640617.txt.
Description
Piyatida Molika and four colleagues compare the protein makeup of cervical cancer cells grown flat versus in balls, in a 2024 paper. Three-dimensional spheroids mimic tumors better than flat cultures and serve drug screening. Yet the three common cell lines form spheroids of very different quality, and the reason was unclear.
Protein profiling showed broadly similar rosters between flat and ball cultures, with key differences in amounts. Only HeLa cells strongly raised the proteins that glue and scaffold a compact sphere: cadherin-binding, cytoskeleton, and adhesion factors. The other two lines, SiHa and C33A, raised them too weakly to pack tightly. The authors read spheroid quality as a remodeling outcome, decided by whether adhesion machinery switches on. Their map tells researchers which line to pick for which question. It also hints at drug targets in the adhesion program itself.
Catalog record
| CREATOR | Piyatida Molika, Kittinun Leetanaporn, Wararat Chiangjong, Pongsakorn Choochuen, Raphatphorn Navakanitworakul |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 53 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |