A High-Throughput Neurosphere-Based Colony Formation Assay to Test Drug and Radiation Sensitivity of Different Patient-Derived Glioblastoma Lines
Manoj Kumar, Lauren C Nassour-Caswell, Hasan Alrefai, Joshua C Anderson, Taylor L Schanel, Patricia H Hicks, Rex Cardan, Christopher D Willey, Mitsuko Masutani · 2024
Read A High-Throughput Neurosphere-Based Colony Formation Assay to Test Drug and Radiation Sensitivity of Different Patient-Derived Glioblastoma Lines by Manoj Kumar, Lauren C Nassour-Caswell, Hasan Alrefai, Joshua C Anderson, Taylor L Schanel, Patricia H Hicks, Rex Cardan, Christopher D Willey, Mitsuko Masutani (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11640616.txt.
Description
Manoj Kumar and colleagues build a fast colony test for glioblastoma drug and radiation response. Their methods paper from twenty twenty-four uses patient-derived tumour spheres. Classic colony assays need weeks in flat cell culture. Sphere models keep tumour stem-like traits that flat cultures lose.
The new assay grows colonies inside neurospheres at high throughput. Drug and radiation sensitivity can be screened across many lines. Patient-derived lines retain the original tumour character better. Radiation death mechanisms are captured broadly by colony outcomes. Serum-free stem media suit these sphere cultures properly. Faster response prediction could personalise glioblastoma treatment trials. The protocol is shared for other labs to adopt.
Catalog record
| CREATOR | Manoj Kumar, Lauren C Nassour-Caswell, Hasan Alrefai, Joshua C Anderson, Taylor L Schanel, Patricia H Hicks, Rex Cardan, Christopher D Willey, Mitsuko Masutani |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 35 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |