A Quadruplex RT-qPCR for the Detection of African Swine Fever Virus, Classical Swine Fever Virus, Porcine Reproductive and Respiratory Syndrome Virus, and Porcine Pseudorabies Virus

Zhuo Feng, Kaichuang Shi, Yanwen Yin, Yuwen Shi, Shuping Feng, Feng Long, Zuzhang Wei, Hongbin Si, Mrigendra Rajput, Neeraj Singh · 2024

Read A Quadruplex RT-qPCR for the Detection of African Swine Fever Virus, Classical Swine Fever Virus, Porcine Reproductive and Respiratory Syndrome Virus, and Porcine Pseudorabies Virus by Zhuo Feng, Kaichuang Shi, Yanwen Yin, Yuwen Shi, Shuping Feng, Feng Long, Zuzhang Wei, Hongbin Si, Mrigendra Rajput, Neeraj Singh (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11640363.txt.

Description

Zhuo Feng and co-authors build a single lab test that spots four pig plagues at once, all with look-alike symptoms. African swine fever, classical swine fever, porcine reproductive and respiratory syndrome, and pseudorabies all bring fever, bleeding, breathing trouble, and abortions. Farms cannot tell them apart by eye, and each demands a different response. Their 2024 assay runs four primer-probe sets in one tube.

Each set homes on a signature gene of one virus. The test detects low copy numbers of each target and cross-reacts with none of the others. One reaction replaces four separate workups. Veterinary labs get a differential diagnosis at the speed outbreaks move.

Catalog record

CREATORZhuo Feng, Kaichuang Shi, Yanwen Yin, Yuwen Shi, Shuping Feng, Feng Long, Zuzhang Wei, Hongbin Si, Mrigendra Rajput, Neeraj Singh
YEAR2024
COLLECTIONPAPERS
FORMATTXT
FILE SIZE40 KB
VERSION1.0
LICENSECC BY 4.0
RIGHTSCC BY
SOURCEEurope PMC

Source: https://europepmc.org/articles/PMC11640363

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