Development and Validation of RAA-CRISPR/Cas12a-Based Assay for Detecting Porcine Rotavirus
Siyu Huang, Longhuan Du, Song Liu, Qingcheng Yang, Changwei Lei, Hongning Wang, Liu Yang, Xin Yang, Fabio Ostanello · 2024
Read Development and Validation of RAA-CRISPR/Cas12a-Based Assay for Detecting Porcine Rotavirus by Siyu Huang, Longhuan Du, Song Liu, Qingcheng Yang, Changwei Lei, Hongning Wang, Liu Yang, Xin Yang, Fabio Ostanello (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11640193.txt.
Description
Siyu Huang and eight co-authors published a 2024 paper on a rapid test for pig rotavirus, a major cause of diarrhoea in piglets. The disease stunts growth and costs the pig industry heavily. The team targeted a gene shared by the two most important virus types so one test could catch both.
Their method joins a fast DNA copying step with CRISPR, a gene-targeting system that releases a fluorescent signal when it finds its target. The test finishes in about thirty minutes at body temperature and needs no large machines. It detected the virus at levels ten times lower than the standard test and ignored five other pig viruses. On farm samples it agreed with the standard test almost perfectly.
Catalog record
| CREATOR | Siyu Huang, Longhuan Du, Song Liu, Qingcheng Yang, Changwei Lei, Hongning Wang, Liu Yang, Xin Yang, Fabio Ostanello |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 36 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |