Hydrogen–Deuterium Exchange Mass Spectrometry Reveals Mechanistic Insights into RNA Oligonucleotide-Mediated Inhibition of TDP-43 Aggregation
Thomas · 2024
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Description
Researchers use in 2024 hydrogen-deuterium exchange mass spectrometry to watch how RNA strands stop a brain protein from clumping. Clumps of TDP-43 mark several degenerative brain diseases. RNA binding is known to block clumping, but the shape changes behind that guard were unknown.
Exchange patterns show RNA shields the protein's RNA-grabbing domains and the linker between them. At high salt, one natural RNA sequence loses its guard while a repeat sequence keeps it. The loss aligns with a salt-driven shape shift in the bound protein. Domain contact changes emerge as the key mechanism. The work may guide RNA-based drugs that keep the protein soluble in diseased brains.
Catalog record
| CREATOR | Thomas |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 52 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |