Practical advice for extracellular vesicle isolation in plant–microbe interactions: Concerns, considerations, and conclusions
Hannah Thieron, Laura Krassini, Seomun Kwon, Sebastian Fricke, Sabrine Nasfi, Lorenz Oberkofler, Alessa Ruf, Julia Kehr, Karl‐Heinz Kogel, Arne Weiberg, Michael Feldbrügge, Silke Robatzek, Ralph Panstruga · 2024
Read Practical advice for extracellular vesicle isolation in plant–microbe interactions: Concerns, considerations, and conclusions by Hannah Thieron, Laura Krassini, Seomun Kwon, Sebastian Fricke, Sabrine Nasfi, Lorenz Oberkofler, Alessa Ruf, Julia Kehr, Karl‐Heinz Kogel, Arne Weiberg, Michael Feldbrügge, Silke Robatzek, Ralph Panstruga (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11635479.txt.
Description
Hannah Thieron and a large consortium offered practical guidance in two thousand twenty-four for isolating tiny membrane bubbles, called extracellular vesicles, in plant and microbe research. These bubbles carry messages between plants and microbes, but plant material brings special pitfalls that animal-cell guides do not cover.
Advice runs step by step through study design, fine-tuning, quality checks, and ways to raise yield and purity while keeping results repeatable. The guidance distills recent papers plus the group's own experience across many plant and microbe systems. Newcomers to the field gain a field-tested starting protocol, and the wider community gains shared standards to compare results.
Catalog record
| CREATOR | Hannah Thieron, Laura Krassini, Seomun Kwon, Sebastian Fricke, Sabrine Nasfi, Lorenz Oberkofler, Alessa Ruf, Julia Kehr, Karl‐Heinz Kogel, Arne Weiberg, Michael Feldbrügge, Silke Robatzek, Ralph Panstruga |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 61 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |