Quantitative Analysis of Kinetochore Protein Levels and Inter-Kinetochore Distances in Mammalian Cells During Mitosis
Neeraj Wasnik, Mahima Singhal, Sukirti Khantwal, Sanghamitra Mylavarapu, Sivaram V S Mylavarapu · 2024
Read Quantitative Analysis of Kinetochore Protein Levels and Inter-Kinetochore Distances in Mammalian Cells During Mitosis by Neeraj Wasnik, Mahima Singhal, Sukirti Khantwal, Sanghamitra Mylavarapu, Sivaram V S Mylavarapu (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11635445.txt.
Description
Neeraj Wasnik, Mahima Singhal, Sukirti Khantwal, Sanghamitra Mylavarapu, and Sivaram Mylavarapu document in a 2024 methods paper how to measure kinetochore protein levels during cell division. The kinetochore is the layered hub where fibers grip chromosomes in mitosis. Its outer proteins arrive early and leave near the split, so their levels mark stage fidelity.
Grip strength between sister units builds the tension that triggers equal segregation. Hundreds of conserved proteins compose the hub, many of them motors and checkpoint guards. The protocols use sharp fluorescence microscopy to count those proteins in place. Sister to sister distances are measured by the same imaging route in the paper. Stage by stage counts at three mitotic phases form the core readout.
Catalog record
| CREATOR | Neeraj Wasnik, Mahima Singhal, Sukirti Khantwal, Sanghamitra Mylavarapu, Sivaram V S Mylavarapu |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 88 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |