Visualization of Bacillus subtilis spore structure and germination using quick-freeze deep-etch electron microscopy
Kiran Jalil, Yuhei O Tahara, Makoto Miyata · 2024
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Description
Kiran Jalil and colleagues freeze bacterial spores in the act of waking up and photograph them in three dimensions, in this study from twenty twenty-four. Bacillus subtilis spores are armored survival pods, hard to image in fine detail. Quick-freeze deep-etch electron microscopy etches a replica of the frozen surface, yielding high-contrast depth.
Five structures came into focus. Fine hairs coat the dormant spore's surface. Spores stripped by enzyme sprout spikes. During germination, the thick cortex layer shows a fractured look as it breaks down. Small vesicles appear to connect with the membrane around the core. And the surface of the newborn vegetative cell keeps changing shape as it emerges. The authors offer the atlas as a structural baseline for spore science, from food safety to sterilization.
Catalog record
| CREATOR | Kiran Jalil, Yuhei O Tahara, Makoto Miyata |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 26 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |