Selective Recruitment of a Synthetic Histone Acetyltransferase Can Boost CHO Cell Productivity
Sienna P. Butterfield, Rebecca E. Sizer, Fay L. Saunders, Robert J. White · 2024
Read Selective Recruitment of a Synthetic Histone Acetyltransferase Can Boost CHO Cell Productivity by Sienna P. Butterfield, Rebecca E. Sizer, Fay L. Saunders, Robert J. White (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11629143.txt.
Description
Sienna Butterfield, Rebecca Sizer, Fay Saunders, and Robert White wrote this 2024 paper on raising output from hamster ovary cells used in drug making. Such CHO cells host most industrial production of biologic drugs. A gene placed in the cell must be switched on hard, and chemical marks on its DNA and histones set how hard it runs.
The team found that the CMV promoter, the standard driver, carries histones with too little acetylation in these cells. They used a CRISPR-based tool to guide an acetyl-adding enzyme part right to that promoter. Antibody message levels rose up to twelvefold. The gain far beat a twin tool that removed methyl marks from the promoter instead. The study shows how targeted epigenetic tweaks, rather than new genes, can lift factory cell output.
Catalog record
| CREATOR | Sienna P. Butterfield, Rebecca E. Sizer, Fay L. Saunders, Robert J. White |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 32 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |