An Improved Transformation‐Associated Recombination Cloning Approach for Direct Capturing of Natural Product Biosynthetic Gene Clusters
Olena Kurylenko, Anja Palusczak, Andriy Luzhetskyy, Yuriy Rebets · 2024
Read An Improved Transformation‐Associated Recombination Cloning Approach for Direct Capturing of Natural Product Biosynthetic Gene Clusters by Olena Kurylenko, Anja Palusczak, Andriy Luzhetskyy, Yuriy Rebets (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11626649.txt.
Description
Bacteria of the Streptomyces family make many of our antibiotics, and the recipes sit in gene clusters up to two hundred thousand letters long. Copying a whole cluster into a test host is the bottleneck for discovering new drugs. Kurylenko, Palusczak, Luzhetskyy, and Rebets improve a yeast-based copying method called TAR cloning. Its old weakness was screening: finding the one yeast colony carrying the right cluster took heavy work.
Their upgrade builds selection into the vector, using the yeast killer phenomenon so only colonies with the target cluster thrive. They prove it by capturing the chelocardin cluster, thirty-five thousand letters, and the daptomycin cluster, sixty-seven thousand. Both produced their antibiotics in a new host. The authors say the direct-selection trick can spread to gene clusters from other bacteria, speeding the hunt for natural products.
Catalog record
| CREATOR | Olena Kurylenko, Anja Palusczak, Andriy Luzhetskyy, Yuriy Rebets |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 41 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |