Molecular validation of genetically transformed Catharanthus roseus plants via different strains of Agrobacterium tumefaciens

Rania El-Tanbouly, Hend Hassan, Lojina M Awd, Azza Ali Makhlouf, Hani G Shalabi, Sarah El-Messeiry · 2024

Read Molecular validation of genetically transformed Catharanthus roseus plants via different strains of Agrobacterium tumefaciens by Rania El-Tanbouly, Hend Hassan, Lojina M Awd, Azza Ali Makhlouf, Hani G Shalabi, Sarah El-Messeiry (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11626052.txt.

Description

Rania El-Tanbouly and co-authors published this study in 2024. It works with Catharanthus roseus, the Madagascar periwinkle, a plant that makes prized cancer and blood pressure drugs. Breeding new plant lines uses a soil bacterium to insert genes, but results vary with the bacterial strain. The team compared three strains carrying a marker gene, inserted by syringe into plant tissue. They then checked the changed plants at the molecular level.

One strain, GV3101, changed the most plants, at about sixty-one percent. Yet its cuttings regrew the worst, at ten percent. Another strain changed fewer plants but regrew them well. Marker gene activity ran highest in the GV3101 plants. DNA fingerprint checks showed wide variation among the new lines. The authors suggest picking the strain to match the goal: change rate or regrowth.

Catalog record

CREATORRania El-Tanbouly, Hend Hassan, Lojina M Awd, Azza Ali Makhlouf, Hani G Shalabi, Sarah El-Messeiry
YEAR2024
COLLECTIONPAPERS
FORMATTXT
FILE SIZE52 KB
VERSION1.0
LICENSECC BY 4.0
RIGHTSCC BY
SOURCEEurope PMC

Source: https://europepmc.org/articles/PMC11626052

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