Protocol for reticulocyte enrichment from low-volume human blood samples from alpha-thalassemic and healthy participants
Stefano Comità, Paola Valentino, Teresa Ceglie, Neha Kargutkar, Harish Kalaiarasan, Giovanni Battista Ferrero, Antonella Roetto · 2024
Read Protocol for reticulocyte enrichment from low-volume human blood samples from alpha-thalassemic and healthy participants by Stefano Comità, Paola Valentino, Teresa Ceglie, Neha Kargutkar, Harish Kalaiarasan, Giovanni Battista Ferrero, Antonella Roetto (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11625235.txt.
Description
Stefano Comità and co-authors published a protocol in 2024 for enriching reticulocytes, immature red blood cells, from low-volume human blood samples. Such isolation is crucial for blood research. It was designed for small samples from healthy people and from people with alpha-thalassemia, an inherited condition that reduces hemoglobin production.
The method has two main stages. First, the blood is centrifuged through two density gradient media that separate cells by weight, yielding a fraction rich in them. Next, a negative magnetic step removes granulocytes and platelets, leaving them purified. The authors also detail how to recover two further cell groups from the original sample: peripheral blood mononuclear cells and mature red blood cells. The enriched cells suit deep multi-omics work on genes and proteins, a big plus when blood volume is scarce.
Catalog record
| CREATOR | Stefano Comità, Paola Valentino, Teresa Ceglie, Neha Kargutkar, Harish Kalaiarasan, Giovanni Battista Ferrero, Antonella Roetto |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 36 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |