Creating Single-Cell Protein-Producing Bacillus subtilis Mutants Using Chemical Mutagen and Amino Acid Inhibitors
Indra Berzina, Martins Kalnins, Zane Geiba, Svetlana Raita, Jelizaveta Palcevska, Taras Mika, Kriss Spalvins · 2024
Read Creating Single-Cell Protein-Producing Bacillus subtilis Mutants Using Chemical Mutagen and Amino Acid Inhibitors by Indra Berzina, Martins Kalnins, Zane Geiba, Svetlana Raita, Jelizaveta Palcevska, Taras Mika, Kriss Spalvins (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11623996.txt.
Description
Indra Berzina and six colleagues published a biotechnology study in two thousand twenty-four on growing protein-rich bacteria. Single-cell protein from microbes could ease food pressure as populations grow and climates shift. The bacterium Bacillus subtilis grows fast and packs protein, making it a good candidate.
The team created mutants with a chemical mutagen, then grew them on media dosed with amino acid blockers. Only bacteria that overproduce protein building blocks could thrive under that pressure. Three herbicide blockers were tuned to doses that stop the normal strain fully. The best mutants, picked under one blocker, gave over seven times more biomass and higher protein content. The authors present the method as a simple route to better producer strains.
Catalog record
| CREATOR | Indra Berzina, Martins Kalnins, Zane Geiba, Svetlana Raita, Jelizaveta Palcevska, Taras Mika, Kriss Spalvins |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 58 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |