Structural basis of deoxynucleotide addition by HIV-1 RT during reverse transcription
Sandra Vergara, Xiaohong Zhou, Ulises Santiago, Mounia Alaoui-El-Azher, James F. Conway, Nicolas Sluis-Cremer, Guillermo Calero · 2024
Read Structural basis of deoxynucleotide addition by HIV-1 RT during reverse transcription by Sandra Vergara, Xiaohong Zhou, Ulises Santiago, Mounia Alaoui-El-Azher, James F. Conway, Nicolas Sluis-Cremer, Guillermo Calero (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11618517.txt.
Description
Sandra Vergara and co-authors published a structural biology study in 2024 on how the HIV enzyme reverse transcriptase adds a DNA building block. The enzyme copies the viral RNA genome into DNA, central to infection. Using active substrates and a rapid-freeze imaging method, the team captured eleven structures spanning the reaction's start, middle, and end states at near-atomic detail.
The opening state shows one metal ion bound and a charged pocket inviting a second. Mid-reaction, the incoming block twists into a new shape that lines up its reactive parts. The product state holds the joined block with its leaving group steadied by metals and bonds. A mutant residue slowed both the enzyme and viral growth.
Catalog record
| CREATOR | Sandra Vergara, Xiaohong Zhou, Ulises Santiago, Mounia Alaoui-El-Azher, James F. Conway, Nicolas Sluis-Cremer, Guillermo Calero |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 62 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |