Allele-tagged TaqMan® PCR genotyping assays for high-throughput detection of soybean cyst nematode resistance
Mariola Usovsky, Kristin Bilyeu, Andrew Bent, Andrew M. Scaboo · 2024
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Description
Mariola Usovsky and co-authors build a quick DNA test panel for soybean resistance to a costly pest. The soybean cyst nematode drains yields, and breeders need fast ways to check which plants carry resistance genes. Whole-genome sequencing gives full detail but is pricey and rigid for routine screening. Their 2024 paper develops targeted TaqMan assays instead.
Starting from a soybean allele catalog, the team picked variants that change the protein output of known resistance genes, including several SNAP genes and a SHMT gene. They turned those variants into a panel of quick PCR tests. The assays were run on elite breeding lines and on crossing populations, and checked against older test formats. Every assay read the alleles cleanly and efficiently. The panel gives breeding programs a cheap, high-throughput screen they can run each season.
Catalog record
| CREATOR | Mariola Usovsky, Kristin Bilyeu, Andrew Bent, Andrew M. Scaboo |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 70 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |