Long-read RNA sequencing of archival tissues reveals novel genes and transcripts associated with clear cell renal cell carcinoma recurrence and immune evasion
Joshua Lee, Elizabeth A. Snell, Joanne Brown, Charlotte E. Booth, Rosamonde E. Banks, Daniel J. Turner, Naveen S. Vasudev, Dimitris Lagos · 2024
Read Long-read RNA sequencing of archival tissues reveals novel genes and transcripts associated with clear cell renal cell carcinoma recurrence and immune evasion by Joshua Lee, Elizabeth A. Snell, Joanne Brown, Charlotte E. Booth, Rosamonde E. Banks, Daniel J. Turner, Naveen S. Vasudev, Dimitris Lagos (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11610457.txt.
Description
Joshua Lee and co-authors use long read RNA sequencing in 2024 on stored kidney tumor tissue to hunt genes tied to cancer return and immune escape. Clear cell renal cancer often comes back after surgery, and stored samples are the main way to study who relapses. Long read methods can spot new gene forms, but they had not been compared head to head in clinic samples.
A dozen stored tumors, half from patients who later relapsed, were read by two long read methods. Results were checked in a second patient group and in a cancer cell line. Stored tissue gave shorter reads than fresh cells, yet both methods agreed on genes that differed in relapse cases. They named a long noncoding gene and a T cell exhaustion marker as relapse candidates, plus many new transcript forms and genes.
Catalog record
| CREATOR | Joshua Lee, Elizabeth A. Snell, Joanne Brown, Charlotte E. Booth, Rosamonde E. Banks, Daniel J. Turner, Naveen S. Vasudev, Dimitris Lagos |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 67 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |