Paradigm of engineering recalcitrant non-model microorganism with dominant metabolic pathway as a biorefinery chassis
Xiongying Yan, Weiwei Bao, Yalun Wu, Chenyue Zhang, Zhitao Mao, Qianqian Yuan, Zhousheng Hu, Penghui He, Qiqun Peng, Mimi Hu, Binan Geng, Hongwu Ma, Shouwen Chen, Qiang Fei, Qiaoning He, Shihui Yang · 2024
Read Paradigm of engineering recalcitrant non-model microorganism with dominant metabolic pathway as a biorefinery chassis by Xiongying Yan, Weiwei Bao, Yalun Wu, Chenyue Zhang, Zhitao Mao, Qianqian Yuan, Zhousheng Hu, Penghui He, Qiqun Peng, Mimi Hu, Binan Geng, Hongwu Ma, Shouwen Chen, Qiang Fei, Qiaoning He, Shihui Yang (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11608335.txt.
Description
Xiongying Yan and fifteen co-authors tame a stubborn bacterium into a factory chassis by deliberately hobbling its favorite pathway. Zymomonas mobilis ferments fast and hard for industry, yet its dominant ethanol route starves rival products. An enzyme-aware genome model first mapped its flux habits. The team then inserted a mild but cofactor-unbalanced butanediol pathway to loosen ethanol's grip, forming an intermediate chassis.
On that chassis a D-lactate strain poured out over 140 grams per liter from glucose, and over 104 from corncob waste liquor, at near-total yield. Cost and life cycle checks supported commercial promise and carbon cuts. The recipe may generalize to other stubborn microbes.
Catalog record
| CREATOR | Xiongying Yan, Weiwei Bao, Yalun Wu, Chenyue Zhang, Zhitao Mao, Qianqian Yuan, Zhousheng Hu, Penghui He, Qiqun Peng, Mimi Hu, Binan Geng, Hongwu Ma, Shouwen Chen, Qiang Fei, Qiaoning He, Shihui Yang |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 75 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |