Structural effects of high laser power densities on an early bacteriorhodopsin photocycle intermediate
Quentin Bertrand, Przemyslaw Nogly, Eriko Nango, Demet Kekilli, Georgii Khusainov, Antonia Furrer, Daniel James, Florian Dworkowski, Petr Skopintsev, Sandra Mous, Isabelle Martiel, Per Börjesson, Giorgia Ortolani, Chia-Ying Huang, Michal Kepa, Dmitry Ozerov, Steffen Brünle, Valerie Panneels, Tomoyuk · 2024
Read Structural effects of high laser power densities on an early bacteriorhodopsin photocycle intermediate by Quentin Bertrand, Przemyslaw Nogly, Eriko Nango, Demet Kekilli, Georgii Khusainov, Antonia Furrer, Daniel James, Florian Dworkowski, Petr Skopintsev, Sandra Mous, Isabelle Martiel, Per Börjesson, Giorgia Ortolani, Chia-Ying Huang, Michal Kepa, Dmitry Ozerov, Steffen Brünle, Valerie Panneels, Tomoyuk (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11603225.txt.
Description
Quentin Bertrand and a large team of co-authors present a 2024 study on whether the intense lasers used to watch proteins work might also distort them. Time-resolved crystallography at X-ray free-electron lasers films molecules mid-reaction, but crystals need strong laser flashes to activate enough molecules. The team collected data on bacteriorhodopsin, a light-driven protein, across an enormous range of laser power, following the energy from picoseconds to milliseconds.
Overall structures barely changed. But at the highest powers the light-sensing molecule's shape shifted measurably and a key cluster of charged groups heated up. The authors compare activation inside crystals with the protein's behavior in solution. They discuss how much of what such experiments see is biology and how much is laser artifact. The work gives the field calibration points for trusting its molecular movies.
Catalog record
| CREATOR | Quentin Bertrand, Przemyslaw Nogly, Eriko Nango, Demet Kekilli, Georgii Khusainov, Antonia Furrer, Daniel James, Florian Dworkowski, Petr Skopintsev, Sandra Mous, Isabelle Martiel, Per Börjesson, Giorgia Ortolani, Chia-Ying Huang, Michal Kepa, Dmitry Ozerov, Steffen Brünle, Valerie Panneels, Tomoyuk |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 47 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |