Development and Optimization of a Redox Enzyme-Based Fluorescence Biosensor for the Identification of MsrB1 Inhibitors
Hyun Bo Shim, Hyunjeong Lee, Hwa Yeon Cho, Young Ho Jo, Lionel Tarrago, Hyunggee Kim, Vadim N Gladyshev, Byung Cheon Lee, Alessandra Napolitano · 2024
Read Development and Optimization of a Redox Enzyme-Based Fluorescence Biosensor for the Identification of MsrB1 Inhibitors by Hyun Bo Shim, Hyunjeong Lee, Hwa Yeon Cho, Young Ho Jo, Lionel Tarrago, Hyunggee Kim, Vadim N Gladyshev, Byung Cheon Lee, Alessandra Napolitano (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11591284.txt.
Description
MsrB1 is a thiol-dependent enzyme that reduces protein methionine-R-sulfoxide and regulates inflammatory response in macrophages. Therefore, MsrB1 could be a promising therapeutic target for the control of inflammation. To identify MsrB1 inhibitors, we construct a redox protein-based fluorescence biosensor composed of MsrB1, a circularly permutated fluorescent protein, and the thioredoxin1 in a single polypeptide chain.
Catalog record
| CREATOR | Hyun Bo Shim, Hyunjeong Lee, Hwa Yeon Cho, Young Ho Jo, Lionel Tarrago, Hyunggee Kim, Vadim N Gladyshev, Byung Cheon Lee, Alessandra Napolitano |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 43 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |