Promoter recognition specificity of Corynebacterium glutamicum stress response sigma factors σD and σH deciphered using computer modeling and point mutagenesis
J. Blumenstein, H. Dostálová, L. Rucká, V. Štěpánek, T. Busche, J. Kalinowski, M. Pátek, I. Barvík · 2024
Read Promoter recognition specificity of Corynebacterium glutamicum stress response sigma factors σD and σH deciphered using computer modeling and point mutagenesis by J. Blumenstein, H. Dostálová, L. Rucká, V. Štěpánek, T. Busche, J. Kalinowski, M. Pátek, I. Barvík (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11588781.txt.
Description
Eight researchers report a 2024 study of how a soil bacterium controls its genes under stress. The work centers on two sigma factors, named sigma D and sigma H. These small parts help the cell start reading certain genes at sites called promoters.
The team mixed lab tests with computer models to see how each factor binds its targets. They changed single building blocks in sigma H and measured the effect on three promoters. The results guided the design of a new mixed promoter. That new promoter drove gene reading well while barely touching the cell's own processes.
Catalog record
| CREATOR | J. Blumenstein, H. Dostálová, L. Rucká, V. Štěpánek, T. Busche, J. Kalinowski, M. Pátek, I. Barvík |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 57 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |