Multiplex Genome Editing of Human Pluripotent Stem Cells Using Cpf1
Haiting Ma · 2024
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Description
Several gene edits can be made in human stem cells in one experiment, Haiting Ma describes in a 2024 protocol. Targeted genome editing of human pluripotent stem cells is critical for basic and translational research. The protocol uses Cpf1, a programmable DNA-cutting enzyme from the CRISPR family that favors AT-rich target sequences, delivered with its guide RNAs from a single vector system.
The procedures enable both small indel formation and precise editing through homologous recombination at multiple loci at once. A single guide array targeted two genes in one experiment, producing a knockin at one locus and a knockout at the other. Edited cells kept pluripotency markers and could differentiate into neurons. A reporter line with a large cassette at the insulin locus was also generated.
Catalog record
| CREATOR | Haiting Ma |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 137 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |