Decreased mitochondrial transcription factor A and mitochondrial DNA copy number promote cyclin-dependent kinase inhibitor 1A expression and reduce tumorigenic properties of colorectal cancer cells
Jessika Buchwaldt, Tania Fritsch, Monika Hartmann, Hagen Roland Witzel, Michael Kloth, Wilfried Roth, Katrin E. Tagscherer, Nils Hartmann · 2024
Read Decreased mitochondrial transcription factor A and mitochondrial DNA copy number promote cyclin-dependent kinase inhibitor 1A expression and reduce tumorigenic properties of colorectal cancer cells by Jessika Buchwaldt, Tania Fritsch, Monika Hartmann, Hagen Roland Witzel, Michael Kloth, Wilfried Roth, Katrin E. Tagscherer, Nils Hartmann (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11586319.txt.
Description
Jessika Buchwaldt and co-authors published this cancer cell study in 2024. Colorectal cancer cells often carry fewer copies of their mitochondrial DNA than healthy cells, and that count tracks patient outcomes. A protein called TFAM controls the count. The team used CRISPR editing to switch TFAM down in a human colorectal cancer cell line. They then sequenced the cells' gene activity and ran growth assays.
With TFAM reduced, mitochondrial DNA copies fell and the genes it encodes quieted. The edited cells also grew more slowly and showed more signs of senescence, the aged state where cells stop dividing. One gene rose sharply: CDKN1A, which makes p21, a brake on cell division. The authors propose p21 as a messenger linking mitochondrial loss to the nucleus. Weakening the cell's power-plant genome, in other words, may push tumor cells toward retirement rather than growth.
Catalog record
| CREATOR | Jessika Buchwaldt, Tania Fritsch, Monika Hartmann, Hagen Roland Witzel, Michael Kloth, Wilfried Roth, Katrin E. Tagscherer, Nils Hartmann |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 39 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |