Bacterial single-cell RNA sequencing captures biofilm transcriptional heterogeneity and differential responses to immune pressure
Lee E. Korshoj, Tammy Kielian · 2024
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Description
Lee Korshoj and Tammy Kielian present a method that reads gene activity in single bacterial cells taken from biofilms. Biofilms are dense cell communities that resist antibiotics and immune attack, and their cells vary widely in state. Their 2024 paper tunes single-cell RNA sequencing for Staphylococcus aureus grown as a biofilm.
The method, called BaSSSh-seq, showed far more cell-to-cell variety in biofilms than in free-floating growth. It mapped gene networks across subpopulations and traced a path from loose cells to biofilm life. After exposure to immune cells, biofilm metabolism, stress response, and virulence genes shifted in distinct ways. Microbiology readers get a sharper tool for finding the cells that help biofilms endure.
Catalog record
| CREATOR | Lee E. Korshoj, Tammy Kielian |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 121 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |