Single-molecule imaging reveals the kinetics of non-homologous end-joining in living cells
Mariia Mikhova, Noah J. Goff, Tomáš Janovič, Joshua R. Heyza, Katheryn Meek, Jens C. Schmidt · 2024
Read Single-molecule imaging reveals the kinetics of non-homologous end-joining in living cells by Mariia Mikhova, Noah J. Goff, Tomáš Janovič, Joshua R. Heyza, Katheryn Meek, Jens C. Schmidt (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11584804.txt.
Description
Mariia Mikhova and co-authors film DNA repair one molecule at a time in a 2024 paper. When both strands of DNA snap, cells mostly fix the break by gluing the ends back together. That gluing path is fast but can scar the code. Until now, no one could watch its steps inside a living cell, so its true speed and order stayed guesswork.
The team built a live-cell imaging method that tracks single repair proteins as they gather at a break. The movies show the repair kit maturing in steps, changing both shape and membership over time. Ends first meet at a distance, then draw close for sealing. Direct timing data like this can guide drugs that jam repair in cancer cells.
Catalog record
| CREATOR | Mariia Mikhova, Noah J. Goff, Tomáš Janovič, Joshua R. Heyza, Katheryn Meek, Jens C. Schmidt |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 84 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |