Single-cell encoded gene silencing for high-throughput combinatorial siRNA screening
Feng Guo, Xianglin Ji, Chuxiao Xiong, Hailiang Sun, Zhenghua Liang, Richard Yan-Do, Baowen Gai, Feng Gao, Linfeng Huang, Zhongping Li, Becki Yi Kuang, Peng Shi · 2024
Read Single-cell encoded gene silencing for high-throughput combinatorial siRNA screening by Feng Guo, Xianglin Ji, Chuxiao Xiong, Hailiang Sun, Zhenghua Liang, Richard Yan-Do, Baowen Gai, Feng Gao, Linfeng Huang, Zhongping Li, Becki Yi Kuang, Peng Shi (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11576956.txt.
Description
Mixing gene-silencing drugs can hit cancer from several sides at once, but finding safe, strong mixes is slow work. Feng Guo and eleven co-authors build a rig that tests such mixes one cell at a time. Their 2024 study uses tiny carriers that answer to light and to magnets, which lets them slip the drugs into single cells with care.
With that rig, the team tested more than 1,300 mixes aimed at genes that drive tumour growth. The best finds used three silencing drugs at once. Two targets stood out as key: a gene that drives cell division and one that helps cells dodge death. Their partners added the extra punch. The rig cuts the time and cost of such screens by a wide margin.
Catalog record
| CREATOR | Feng Guo, Xianglin Ji, Chuxiao Xiong, Hailiang Sun, Zhenghua Liang, Richard Yan-Do, Baowen Gai, Feng Gao, Linfeng Huang, Zhongping Li, Becki Yi Kuang, Peng Shi |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 69 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |