Development of a Tagmentation‐Based Next‐Generation Sequencing Clinical Assay as an Alternative to Capillary Electrophoresis‐Based Sequencing
Wei Cheng David Kuek, Chean Nee Chai, Wei Ming Jason Tham, Alvin Yu Jin Ng, Dilys Shi Ning Lau, Janice Yen Qi Loo, Dan Thu Van, Joanna Kia Min Tan, Chun Kiat Lee, Benedict Yan, Tim Hon Man Chan · 2024
Read Development of a Tagmentation‐Based Next‐Generation Sequencing Clinical Assay as an Alternative to Capillary Electrophoresis‐Based Sequencing by Wei Cheng David Kuek, Chean Nee Chai, Wei Ming Jason Tham, Alvin Yu Jin Ng, Dilys Shi Ning Lau, Janice Yen Qi Loo, Dan Thu Van, Joanna Kia Min Tan, Chun Kiat Lee, Benedict Yan, Tim Hon Man Chan (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11574739.txt.
Description
BackgroundNext‐generation sequencing (NGS) technology enables sample multiplexing for interrogation of multiple regions of interest (ROI). Leveraging this, together with access to affordable NGS platforms, we explored the practicality of moving capillary electrophoresis (CE), noncapillary electrophoresis and single‐gene testing to NGS. In this work, we evaluated the iSeq 100's capacity to validate 89 samples at once.
Catalog record
| CREATOR | Wei Cheng David Kuek, Chean Nee Chai, Wei Ming Jason Tham, Alvin Yu Jin Ng, Dilys Shi Ning Lau, Janice Yen Qi Loo, Dan Thu Van, Joanna Kia Min Tan, Chun Kiat Lee, Benedict Yan, Tim Hon Man Chan |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 26 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |