Patch-walking, a coordinated multi-pipette patch clamp for efficiently finding synaptic connections
Mighten C Yip, Mercedes M Gonzalez, Colby F Lewallen, Corey R Landry, Ilya Kolb, Bo Yang, William M Stoy, Ming-fai Fong, Matthew JM Rowan, Edward S Boyden, Craig R Forest, Jeffrey C Smith, John R Huguenard, Jeffrey C Smith, Anonymous, Anonymous, Anonymous, Mighten C Yip, Mercedes M Gonzalez, Colby F · 2024
Read Patch-walking, a coordinated multi-pipette patch clamp for efficiently finding synaptic connections by Mighten C Yip, Mercedes M Gonzalez, Colby F Lewallen, Corey R Landry, Ilya Kolb, Bo Yang, William M Stoy, Ming-fai Fong, Matthew JM Rowan, Edward S Boyden, Craig R Forest, Jeffrey C Smith, John R Huguenard, Jeffrey C Smith, Anonymous, Anonymous, Anonymous, Mighten C Yip, Mercedes M Gonzalez, Colby F (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11573346.txt.
Description
Mapping which neurons connect to which is slow when every electrode must be pulled and cleaned between tries. Mighten Yip and co-authors present patch-walking: with several pipettes in the tissue, only one gets cleaned and moved at a time, like a step forward. Each fresh recording can test connections against all the cells already held.
In mouse brain slices, the team made 136 attempts and landed seventy-one whole-cell recordings. They probed twenty-nine pairs of cells and found three connections. Against the classic method, patch-walking yields eighty to ninety-two percent more tested connections in typical experiment sizes. The 2024 paper makes connectivity hunts far more efficient.
Catalog record
| CREATOR | Mighten C Yip, Mercedes M Gonzalez, Colby F Lewallen, Corey R Landry, Ilya Kolb, Bo Yang, William M Stoy, Ming-fai Fong, Matthew JM Rowan, Edward S Boyden, Craig R Forest, Jeffrey C Smith, John R Huguenard, Jeffrey C Smith, Anonymous, Anonymous, Anonymous, Mighten C Yip, Mercedes M Gonzalez, Colby F |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 46 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |