Precise genetic engineering with piggyBac transposon in plants
Ayako Nishizawa-Yokoi, Seiichi Toki · 2023
Read Precise genetic engineering with piggyBac transposon in plants by Ayako Nishizawa-Yokoi, Seiichi Toki (2023) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10905368.txt.
Description
Ayako Nishizawa-Yokoi and Seiichi Toki review a gene editing tool for plants in a 2023 paper. The tool is a transposon called piggyBac, a jumping gene first found in a moth. Jumping genes move within a genome and have long served as engineering tools. The element has a rare virtue: when it jumps out, it leaves the site clean, with no scar in the DNA.
The authors sum up their work applying that virtue in rice. They showed the element can be cut out of an inserted gene with precision. Combined with targeted repair, it lets breeders place a single desired letter change in a gene, then remove all helper DNA. They also designed a system that briefly expresses cutting enzymes, then erases itself. That suits crops grown from cuttings, where leftover foreign DNA cannot be bred away.
Catalog record
| CREATOR | Ayako Nishizawa-Yokoi, Seiichi Toki |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 25 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |