Scaling up production of recombinant human basic fibroblast growth factor in an Escherichia coli BL21(DE3) plysS strain and evaluation of its pro-wound healing efficacy
Le Li, Bingjie Yu, Yingji Lai, Siyuan Shen, Yawei Yan, Guojun Dong, Xiangyun Gao, Yanrong Cao, Caojie Ge, Liqin Zhu, Huan Liu, Shanhui Tao, Zhiang Yao, Shijun Li, Xiaojie Wang, Qi Hui · 2024
Read Scaling up production of recombinant human basic fibroblast growth factor in an Escherichia coli BL21(DE3) plysS strain and evaluation of its pro-wound healing efficacy by Le Li, Bingjie Yu, Yingji Lai, Siyuan Shen, Yawei Yan, Guojun Dong, Xiangyun Gao, Yanrong Cao, Caojie Ge, Liqin Zhu, Huan Liu, Shanhui Tao, Zhiang Yao, Shijun Li, Xiaojie Wang, Qi Hui (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10875678.txt.
Description
Le Li and a large team scale up production of a human growth factor used in wound healing research and care, in work published in 2023. The protein, basic fibroblast growth factor, drives many cell processes and is in high demand. The team builds a 500 liter fermentation and purification process in a common bacterial host.
Growth conditions were tuned with a statistical design method and then validated at full scale. A three step column purification yields the mature protein, whose identity was confirmed by several analytic methods. Activity tests in cells and in a diabetic wound model in animals check healing effect. The process aims to supply research and clinical needs at larger volume with consistent quality.
Catalog record
| CREATOR | Le Li, Bingjie Yu, Yingji Lai, Siyuan Shen, Yawei Yan, Guojun Dong, Xiangyun Gao, Yanrong Cao, Caojie Ge, Liqin Zhu, Huan Liu, Shanhui Tao, Zhiang Yao, Shijun Li, Xiaojie Wang, Qi Hui |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 49 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |