Characterization of CD4+ and CD8+ T cells responses in the mixed lymphocyte reaction by flow cytometry and single cell RNA sequencing
Adèle Mangelinck, Agathe Dubuisson, Etienne Becht, Sandra Dromaint-Catesson, Manon Fasquel, Nicolas Provost, Dawid Walas, Hélène Darville, Jean-Pierre Galizzi, Céline Lefebvre, Véronique Blanc, Vincent Lombardi · 2024
Read Characterization of CD4+ and CD8+ T cells responses in the mixed lymphocyte reaction by flow cytometry and single cell RNA sequencing by Adèle Mangelinck, Agathe Dubuisson, Etienne Becht, Sandra Dromaint-Catesson, Manon Fasquel, Nicolas Provost, Dawid Walas, Hélène Darville, Jean-Pierre Galizzi, Céline Lefebvre, Véronique Blanc, Vincent Lombardi (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10820991.txt.
Description
Adèle Mangelinck and eleven co-authors mapped how two kinds of T cells respond in a standard lab test, in twenty twenty-three. The test mixes immune cells from two donors and is widely used to judge immune drugs. The team tracked CD4 and CD8 T cells with flow tools, protein assays, and single-cell gene reads over time.
The two kinds shared classic signs of response, such as growth and raised output. But they differed in timing, strength, and the signals they released. Three new markers stood out in the reacting cells. Gene reads mapped cell talk at the contact point and pathways switched on downstream. The authors say the map can help judge drugs and point to new immune therapy ideas.
Catalog record
| CREATOR | Adèle Mangelinck, Agathe Dubuisson, Etienne Becht, Sandra Dromaint-Catesson, Manon Fasquel, Nicolas Provost, Dawid Walas, Hélène Darville, Jean-Pierre Galizzi, Céline Lefebvre, Véronique Blanc, Vincent Lombardi |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 50 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |