Influence of the mRNA initial region on protein production: a case study using recombinant detoxified pneumolysin as a model
Filipe Fusco, Manuella Cazelato Pires, Alexandre Paulo Yague Lopes, Vítor dos Santos Alves, Viviane Maimoni Gonçalves · 2024
Read Influence of the mRNA initial region on protein production: a case study using recombinant detoxified pneumolysin as a model by Filipe Fusco, Manuella Cazelato Pires, Alexandre Paulo Yague Lopes, Vítor dos Santos Alves, Viviane Maimoni Gonçalves (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10800503.txt.
Description
Filipe Fusco and co-authors show that the first few letters of a gene's message can decide how much protein a factory cell makes. Recombinant proteins, many of them medicines, are chronically underproduced, and engineers usually blame the bioreactor or the purification train. Their paper, published in 2023, instead inspects the start of the messenger RNA in bacteria making a detoxified pneumolysin, a vaccine candidate protein.
Clones with and without a small purification tag at the protein's head were grown in ten-liter reactors, alongside a version of the gene whose opening letters were silently rewritten. The tag's presence raised output at least one and a half times, and computer folding of the message explained why: its opening formed a structure the ribosome opens easily. The rewritten version folded tighter and produced less. The authors plead for molecular design to join process design in yield work.
Catalog record
| CREATOR | Filipe Fusco, Manuella Cazelato Pires, Alexandre Paulo Yague Lopes, Vítor dos Santos Alves, Viviane Maimoni Gonçalves |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 45 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |