How to measure your microscope’s HPF. A critical guide for residents

Salvatore Lorenzo Renne · 2023

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Description

Salvatore Lorenzo Renne writes a practical guide for pathology residents in a 2023 paper. Counting cells under the microscope is daily work, and reports often give counts per high-power field. The catch: that field's area differs from microscope to microscope, so counts are not portable between labs.

Renne shows two fixes: calibrate with a tiny ruler slide, or compute the field's diameter from the eyepiece and lens numbers printed on the scope. His worked example turns a common pairing into an area in square millimetres. The guide also asks how far off a trainee's count can drift when the field size is wrong, a quiet source of diagnostic error.

Catalog record

CREATORSalvatore Lorenzo Renne
YEAR2023
COLLECTIONPAPERS
FORMATTXT
FILE SIZE15 KB
VERSION1.0
LICENSECC BY 4.0
RIGHTSCC BY
SOURCEEurope PMC

Source: https://europepmc.org/articles/PMC10767800

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