Precise base editing without unintended indels in human cells and mouse primary myoblasts
Da Eun Yoon, Na-Rae Kim, Soo-Ji Park, Tae Yeong Jeong, Bokkee Eun, Yongcheol Cho, Soo-Yeon Lim, Hyunji Lee, Je Kyoung Seong, Kyoungmi Kim · 2023
Read Precise base editing without unintended indels in human cells and mouse primary myoblasts by Da Eun Yoon, Na-Rae Kim, Soo-Ji Park, Tae Yeong Jeong, Bokkee Eun, Yongcheol Cho, Soo-Yeon Lim, Hyunji Lee, Je Kyoung Seong, Kyoungmi Kim (2023) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10766602.txt.
Description
Da Eun Yoon and co-authors reported in 2023 on making base editing cleaner. Base editors change single DNA letters with great power, but they often cause unwanted insertions and deletions at the target site. Those indels are a real barrier for clinical use. The team set out to remove them while keeping the edits efficient.
Their fix swaps the nickase Cas9 in standard editors for dead Cas9, which cannot cut. They also added chromatin-modulating peptides to raise the conversion rate. In human cell lines and mouse primary myoblasts, the new setup gave accurate substitutions without the unwanted indels. It also showed fewer off-target effects at the DNA level than standard editors. The authors see it as a step toward safer gene-editing therapies.
Catalog record
| CREATOR | Da Eun Yoon, Na-Rae Kim, Soo-Ji Park, Tae Yeong Jeong, Bokkee Eun, Yongcheol Cho, Soo-Yeon Lim, Hyunji Lee, Je Kyoung Seong, Kyoungmi Kim |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 38 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |