Protocol for generation of humanized HCC mouse model and cancer-driver mutations using CRISPR-Cas9
Yicheng Zhu, Stanley M Tahara, Hidekazu Tsukamoto, Keigo Machida · 2023
Read Protocol for generation of humanized HCC mouse model and cancer-driver mutations using CRISPR-Cas9 by Yicheng Zhu, Stanley M Tahara, Hidekazu Tsukamoto, Keigo Machida (2023) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10751556.txt.
Description
Yicheng Zhu, Stanley Tahara, Hidekazu Tsukamoto, and Keigo Machida wrote this 2023 protocol for building a humanized mouse model of liver cancer. Hepatocellular carcinoma is the main form of liver cancer. To study it, the team made mice whose liver and blood cells are partly human.
The recipe humanizes three cell groups: the liver's main cells, its support cells, and its blood-forming cells. Gene editing with CRISPR-Cas9 then adds cancer-driver changes found in patients whose cancer grew from metabolic liver disease. One gene, ARID1A, is knocked out, and another, CTNNB1, is switched on for good. An alcohol-rich Western diet adds the environmental push. The model lets researchers watch how genes and lifestyle join forces to start the cancer. Full steps point to an earlier paper by Yeh and co-authors.
Catalog record
| CREATOR | Yicheng Zhu, Stanley M Tahara, Hidekazu Tsukamoto, Keigo Machida |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 340 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |