Establishment of a Rapid and Effective Agrobacterium-Mediated Genetic Transformation System of Oxalis triangularis ‘Purpurea’
Yun Xiao, Wanli Tuo, Xuexuan Wang, Baomin Feng, Xinyu Xu, Sagheer Ahmad, Junwen Zhai, Donghui Peng, Shasha Wu, Myeong-Je Cho · 2023
Read Establishment of a Rapid and Effective Agrobacterium-Mediated Genetic Transformation System of Oxalis triangularis ‘Purpurea’ by Yun Xiao, Wanli Tuo, Xuexuan Wang, Baomin Feng, Xinyu Xu, Sagheer Ahmad, Junwen Zhai, Donghui Peng, Shasha Wu, Myeong-Je Cho (2023) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10747433.txt.
Description
Yun Xiao and co-authors set up a fast, reliable way to insert new genes into a popular ornamental plant, the purple false shamrock. Their 2023 study notes the plant's landscaping value and the need to learn what its genes do. Without a working transformation method, that gene work stalls, so the team tuned each step of the standard bacterium-based method.
They varied leaf material, growing times, chemical doses, and infection times. The best recipe used leaf discs grown for three days, a set chemical dose, and an eleven-minute infection. It yielded new plants in 79 days, with a success rate near ten percent. The protocol is simple and quick.
Catalog record
| CREATOR | Yun Xiao, Wanli Tuo, Xuexuan Wang, Baomin Feng, Xinyu Xu, Sagheer Ahmad, Junwen Zhai, Donghui Peng, Shasha Wu, Myeong-Je Cho |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 45 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |