Potential of the Novel Slot Blot Method with a PVDF Membrane for Protein Identification and Quantification in Kampo Medicines
Takanobu Takata, Togen Masauji, Yoshiharu Motoo, Laura Donato · 2023
Read Potential of the Novel Slot Blot Method with a PVDF Membrane for Protein Identification and Quantification in Kampo Medicines by Takanobu Takata, Togen Masauji, Yoshiharu Motoo, Laura Donato (2023) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10745123.txt.
Description
Takanobu Takata and co-authors develop a lab method for finding proteins in Japanese herbal medicines in a 2023 paper. Kampo, Japan's adaptation of traditional Chinese medicine, blends crude plant and other drugs whose full makeup is unknown. Their proteins are scarce and wash out in watery solvents, which makes them hard to identify and count. Yet dose and safety work needs those numbers.
Their method rests on membrane chromatography with a plastic PVDF membrane that grabs proteins. A slot blot format built on the same idea has a further perk: the sample volume is not capped, so dilute proteins can be loaded until they register. The paper weighs the method's potential for naming and measuring proteins in Kampo formulas. Better protein data could tighten quality control of these widely used medicines.
Catalog record
| CREATOR | Takanobu Takata, Togen Masauji, Yoshiharu Motoo, Laura Donato |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 33 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |