Isothermal Amplification and CRISPR/Cas12a-System-Based Assay for Rapid, Sensitive and Visual Detection of Staphylococcus aureus
Danhong Xu, Haijuan Zeng, Wenhui Wu, Hua Liu, Jinbin Wang, Michael Tunick · 2023
Read Isothermal Amplification and CRISPR/Cas12a-System-Based Assay for Rapid, Sensitive and Visual Detection of Staphylococcus aureus by Danhong Xu, Haijuan Zeng, Wenhui Wu, Hua Liu, Jinbin Wang, Michael Tunick (2023) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10742561.txt.
Description
Xu and co-authors build a quick, eyes-on test for a common food-poisoning germ. Staphylococcus aureus spreads widely and can cause dangerous blood infections, so food checks need speed and accuracy. Their platform first copies the germ's nuc gene at constant temperature, then uses a CRISPR protein that lights up or marks a test strip when the target is present.
On test strips, the method spots vanishingly small amounts of the gene and a few hundred bacteria per milliliter; a fluorescence version goes ten times lower. The whole check takes about forty minutes and works in dairy products. The authors present it as a low-cost field tool for food safety.
Catalog record
| CREATOR | Danhong Xu, Haijuan Zeng, Wenhui Wu, Hua Liu, Jinbin Wang, Michael Tunick |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 30 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |