Bone Formation in Zebrafish: The Significance of DAF-FM DA Staining for Nitric Oxide Detection
Ann Huysseune, Ulrike G Larsen, Daria Larionova, Cecilie L Matthiesen, Steen V Petersen, Marc Muller, P Eckhard Witten, Enrico Moro · 2023
Read Bone Formation in Zebrafish: The Significance of DAF-FM DA Staining for Nitric Oxide Detection by Ann Huysseune, Ulrike G Larsen, Daria Larionova, Cecilie L Matthiesen, Steen V Petersen, Marc Muller, P Eckhard Witten, Enrico Moro (2023) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10742054.txt.
Description
Ann Huysseune and co-authors present a 2023 study on a fluorescent stain used to watch bones form in zebrafish embryos. The stain, DAF-FM DA, is meant to reveal nitric oxide, a signaling gas, and in fish it lights up exactly where bone first appears. Where precisely the glow sits inside the tissue, though, had never been pinned down.
Sectioning stained embryos, the team found the signal sat in the bone and tooth matrix, the notochord sheath, and a heart vessel, and always outside cells, even after brief staining. The glow marked forming bone whether or not it had mineralized yet. Blocking nitric oxide production still prevented bones from forming at all. The stain remains a fine live marker of bone formation, the authors conclude, but not a window on the gas inside cells.
Catalog record
| CREATOR | Ann Huysseune, Ulrike G Larsen, Daria Larionova, Cecilie L Matthiesen, Steen V Petersen, Marc Muller, P Eckhard Witten, Enrico Moro |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 36 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |