Competitive Specific Anchorage of Molecules onto Surfaces: Quantitative Control of Grafting Densities and Contamination by Free Anchors
Oksana Kirichuk, Sumitra Srimasorn, Xiaoli Zhang, Abigail R E Roberts, Liliane Coche-Guerente, Jessica C F Kwok, Lionel Bureau, Delphine Débarre, Ralf P Richter · 2023
Read Competitive Specific Anchorage of Molecules onto Surfaces: Quantitative Control of Grafting Densities and Contamination by Free Anchors by Oksana Kirichuk, Sumitra Srimasorn, Xiaoli Zhang, Abigail R E Roberts, Liliane Coche-Guerente, Jessica C F Kwok, Lionel Bureau, Delphine Débarre, Ralf P Richter (2023) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10734310.txt.
Description
Building surfaces coated with proteins or sugars at exact densities is a basic need for lab-made membranes and binding tests. Oksana Kirichuk and co-authors give a recipe for dialing that density up or down in a 2023 paper.
Molecules carry an anchor that sticks to a surface packed with anchor sites. By mixing the target molecules with loose free anchors in set ratios, the team can preset how many targets land. They back the recipe with theory that links the mix to the final density, and prove it with the common biotin anchor across many densities and molecule sizes. Flipped around, the same trick can measure leftover free anchor in a sample, a handy purity check.
Catalog record
| CREATOR | Oksana Kirichuk, Sumitra Srimasorn, Xiaoli Zhang, Abigail R E Roberts, Liliane Coche-Guerente, Jessica C F Kwok, Lionel Bureau, Delphine Débarre, Ralf P Richter |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 70 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |