De Novo Synthesis of a Conjugative System from Human Gut Metagenomic Data for Targeted Delivery of Cas9 Antimicrobials
Thomas · 2023
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Description
Thomas Hamilton and seven co-authors rebuild a bacterial mating system from gut DNA scraps to deliver gene editors. Their study, from twenty twenty-three, mines human gut metagenomes. The team hunts the discarded contigs that standard pipelines skip. Over fifteen hundred carry conjugation genes, unevenly spread across people.
One full system, from a Citrobacter species, is chosen as scaffold. Its fifty-four kilobase span, with at least forty-seven genes, is synthesized from scratch. The built plasmid moves between bacteria as hoped. It transfers into its target species thirty times more often than back into the donor. Such couriers could one day deliver Cas9 antimicrobials precisely.
Catalog record
| CREATOR | Thomas |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 45 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |