Nanopore adaptive sampling enriches for antimicrobial resistance genes in microbial communities
Danielle C. Wrenn, Devin M. Drown, Devin Drown, Devin Drown · 2023
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Description
Danielle Wrenn and Devin Drown test smart sequencing to enrich resistance genes, in work published in 2023. Antimicrobial resistance threatens public health worldwide, and wild microbe groups act as gene reservoirs. Watching those reservoirs could warn how resistance spreads, but target genes are rare in mixed samples.
Nanopore sequencing with adaptive sampling rejects off target strands in real time during a run. Tests used a mock group of environmental origin and a compact portable setup. Target share rose about four fold on average with adaptive sampling on. Total output fell, yet target yield rose in most repeat runs. A real environmental sample also showed enrichment, which supports fast field surveillance use.
Catalog record
| CREATOR | Danielle C. Wrenn, Devin M. Drown, Devin Drown, Devin Drown |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 38 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |