Integration of xeno-free single-cell cloning in CRISPR-mediated DNA editing of human iPSCs improves homogeneity and methodological efficiency of cellular disease modeling
Atefeh Namipashaki, Kealan Pugsley, Xiaodong Liu, Kirra Abrehart, Sue Mei Lim, Guizhi Sun, Marco J Herold, Jose M Polo, Mark A Bellgrove, Ziarih Hawi · 2023
Read Integration of xeno-free single-cell cloning in CRISPR-mediated DNA editing of human iPSCs improves homogeneity and methodological efficiency of cellular disease modeling by Atefeh Namipashaki, Kealan Pugsley, Xiaodong Liu, Kirra Abrehart, Sue Mei Lim, Guizhi Sun, Marco J Herold, Jose M Polo, Mark A Bellgrove, Ziarih Hawi (2023) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10724053.txt.
Description
Atefeh Namipashaki and co-authors pair gene editing of stem cells with a clean single-cell cloning method. Their 2023 paper targets disease modeling with induced pluripotent stem cells. Edited cells usually grow in mixed crowds or on feeder layers, forcing slow screening to purify the right clones.
Their animal-free method lifts clone survival to up to seventy percent within ten days. Better cell survival pairs with an enriched setting for lone cells to take hold. Transfer rates top ninety-seven percent in their runs. Edit rates reach about half to two-thirds for cutting repairs and a tenth for precise rewrites. The team presents the method as a faster, cleaner route to uniform edited stem cell lines for disease study.
Catalog record
| CREATOR | Atefeh Namipashaki, Kealan Pugsley, Xiaodong Liu, Kirra Abrehart, Sue Mei Lim, Guizhi Sun, Marco J Herold, Jose M Polo, Mark A Bellgrove, Ziarih Hawi |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 42 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |