Deep in situ microscopy for real-time analysis of mammalian cell populations in bioreactors
Jean-Sébastien Guez, Pierre-Yves Lacroix, Thierry Château, Christophe Vial · 2023
Read Deep in situ microscopy for real-time analysis of mammalian cell populations in bioreactors by Jean-Sébastien Guez, Pierre-Yves Lacroix, Thierry Château, Christophe Vial (2023) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-10716407.txt.
Description
Jean-Sébastien Guez and co-authors pair a microscope inside a bioreactor with AI that sorts cells as they grow. Drug makers grow mammalian cells to produce antibodies, and knowing how many cells are alive, dying, or dead shapes each run. Their 2023 paper built a database of nearly a million images from a lab-scale antibody process.
A convolutional network learned to class cells in real time: viable, necrotic, and apoptotic. The system tracked how classes shifted when cells were starved, overfed, or starved of oxygen. It even flagged a class of healthy cells with surface bulges under feast and famine swings. Bioprocess engineers get live cell census data without drawing samples.
Catalog record
| CREATOR | Jean-Sébastien Guez, Pierre-Yves Lacroix, Thierry Château, Christophe Vial |
|---|---|
| YEAR | 2023 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 54 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |